Rui-Shan Wu 
,
En-Hui Qiu,
Jia-Ji Zhu
For correspondence:- Rui-Shan Wu Email: wuruishanrs@hotmail.com Tel:+8659522768177
Received: 30 June 2016 Accepted: 13 March 2017 Published: 30 April 2017
Citation: Wu R, Qiu E, Zhu J. Cynodon dactylon (L) Pers (Poaceae) root extract induces apoptotic cell death via the cyclin D1 pathway in human nasopharyngeal carcinoma cells in vitro and in vivo. Trop J Pharm Res 2017; 16(4):897-903 doi: 10.4314/tjpr.v16i4.22
© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the activity of Cynodon dactylon root extract as a potential anticancer agent.
Methods: Human nasopharyngeal carcinoma HNK-1 cells were treated with ethanol extract of C. dactylon roots. After treatment with C. dactylon root extract, cells were assessed for their cytotoxicity, metabolic status, apoptosis and cell cycle progression. The anticancer activity of the extract was also determined in vivo using a mouse model.
Results: C. dactylon extract exhibited significant cytotoxicity and arrested cell cycle in HNK-1 cells in a dose-dependent manner. Fluorescence-activated cell sorting (FACS) data revealed that it also induced apoptotic cell death. Rb phosphorylation and mRNA levels of cyclin E were significantly reduced. Moreover, C. dactylon extract caused a significant reduction in tumor size and volume in vivo.
Conclusion: C. dactylon root extract induces apoptosis in nasopharyngeal cancer cells via cyclin D1 pathway and inhibits tumor growth in vivo
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